Development of an Antimicrobial Peptide SAAP‐148‐Functionalized Supramolecular Coating on Titanium to Prevent Biomaterial‐Associated Infections

Titanium implants are widely used in medicine but have a risk of biomaterial-associated infection (BAI), which traditional antibiotic-based treatment is affected by resistance. Antimicrobial peptides (AMPs) to successfully kill antibiotic-resistant bacteria. Herein, supramolecular coating for titanium developed presents the synthetic antimicrobial and antibiofilm peptide SAAP-148 via interactions using ureido-pyrimidinone units (UPy-SAAP-148GG). Material characterization dropcast coatings shows presence UPy-SAAP-148GG at surface. The immobilized remains antimicrobially active polymer films can (antibiotic-resistant) Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli. Minor toxicity human dermal fibroblasts observed, with reduced cell attachment after 24 h. Subsequently, dipcoat on tested vivo subcutaneous implant mouse model S. aureus administered locally before implantation mimic contamination during surgery. containing 5 mol% significantly prevents colonization surface as well surrounding tissue, no signs toxicity. This that AMP eminently suitable prevent BAI.